本卷涵盖了用于发现核孔复合体（NPC）的组成、结构和动力学的所有方法，以及转运过程中涉及的可溶性转运因子。各章详细介绍了结构生物学方法、生物物理学、运输因子以及多年来使用的各种模式生物。以分子生物学系列中非常成功的方法的形式编写，每章包括对主题的介绍，列出必要的材料和试剂，包括故障排除和已知陷阱的提示，以及一步一步、易于复制的方案。
The Nuclear Pore Complex
This volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble transport factors involved in the transport process. Chapters detail structural biology methods, biophysical, transport factors , and a wide range of model organisms that have been used over the years. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
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